Substrate‐dependent inhibition of protein kinase C by specific inhibitors
- 9 April 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 263 (1) , 169-171
- https://doi.org/10.1016/0014-5793(90)80731-w
Abstract
Protein kinase C (PKC) and its proteolysis-derived protein kinase independent of Ca2+ and phospholipids (PKM), were purified from rat brain. By using histone HI and protamine as substrates, we assayed the effect of several inhibitors of PKC and PKM. The inhibition turned out to be dependent on both the nature of the kinase and the type of substrate assayed. These results may help to interpret the different responses elicited by PKC inhibitors in vivo.This work was supported by Grant PM880025 from CICYT (Spain).Peer RevieweKeywords
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