CANGS: a user-friendly utility for processing and analyzing 454 GS-FLX data in biodiversity studies
Open Access
- 1 January 2010
- journal article
- Published by Springer Nature in BMC Research Notes
- Vol. 3 (1) , 3
- https://doi.org/10.1186/1756-0500-3-3
Abstract
Next generation sequencing (NGS) technologies have substantially increased the sequence output while the costs were dramatically reduced. In addition to the use in whole genome sequencing, the 454 GS-FLX platform is becoming a widely used tool for biodiversity surveys based on amplicon sequencing. In order to use NGS for biodiversity surveys, software tools are required, which perform quality control, trimming of the sequence reads, removal of PCR primers, and generation of input files for downstream analyses. A user-friendly software utility that carries out these steps is still lacking.Keywords
This publication has 7 references indexed in Scilit:
- Microbial Population Structures in the Deep Marine BiosphereScience, 2007
- Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencingNature Medicine, 2006
- FastGroupII: A web-based bioinformatics platform for analyses of large 16S rDNA librariesBMC Bioinformatics, 2006
- Introducing DOTUR, a Computer Program for Defining Operational Taxonomic Units and Estimating Species RichnessApplied and Environmental Microbiology, 2005
- MAFFT version 5: improvement in accuracy of multiple sequence alignmentNucleic Acids Research, 2005
- The Bioperl Toolkit: Perl Modules for the Life SciencesGenome Research, 2002
- Basic local alignment search toolJournal of Molecular Biology, 1990