STUDY OF LYMPHOCYTE SUBPOPULATIONS IN NORMAL HUMANS AND PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS BY FRACTIONATION OF PERIPHERAL-BLOOD LYMPHOCYTES ON A DISCONTINUOUS FICOLL GRADIENT

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 26  (2) , 228-238
Abstract
Peripheral blood lymphocytes of 30 normal volunteers and 52 patients with systemic lupus erythematous [SLE] were fractionated using a discontinuous (5-30%) Ficoll gradient. Such fractionation permitted the isolation, identification and study of null cells, T [thymus-derived] cells and B [bone marrow-derived] cells. Patients with inactive SLE had a cell distribution and responsiveness to PHA [phytohemagglutinin], Con A [concanavalin A] and pokeweed mitogen (PWM) similar to controls. Patients with active SLE showed a significant decrease in T-cell fractions and a relative increase in null cells, a normal distribution of B cells, a marked reduction in responsiveness to Con A, a lesser reduction to PHA and only a minor reduction to PWM. With increasing disease activity, the number of null cells increased despite lymphopenia. Spontaneous lymphocyte transformation was observed in patients with SLE. This occurred predominantly in the fractions enriched in B cells and was observed early (0-16 h) and late 68-72 h) in the lymphocyte cultures. The method of discontinuous Ficoll gradients is versatile aand reproducible, with good correlations between isolated lymphoid subpopulations and disease activity.