Amphotericin B resistance is recessive in Chinese hamster hybrid cells
- 1 January 1981
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 106 (1) , 41-47
- https://doi.org/10.1002/jcp.1041060106
Abstract
Previous studies from our laboratory have shown that Chinese hamster V79 cells mutated to high level resistance to amphotericin B have a lower cellular level of cholesterol, the target molecule for the polyene antibiotic. Two amphotericin B-resistant (AMBR) mutants were each hybridized to their parental amphotericin B-sensitive (AMBS) V79 cells. All the hybrids derived from AMBR/AMBS fusions were as sensitive to polyene antibiotics (amphotericin B, filipin, and pimaricin) as AMBS/AMBS hybrids. The AMBR/AMBS hybrids were found to contain cholesterol per phospholipids that is comparable to those in AMBS or AMBS/AMBS. The analysis of hybrids formed between mutant and wild-type cells thus indicated that resistance to amphotericin B is a recessive marker, and that the cellular level of cholesterol is compensated in the AMBS/AMBR hybrids. Hybrids of AMBR and AMBR cells were all resistant, so that the three AMBR mutants all fell into a single complementation group.This publication has 16 references indexed in Scilit:
- Growth of amphotericin B-resistant hamster cell line requires exogenous cholesterolExperimental Cell Research, 1980
- Polyene macrolide antibiotic cytotoxicity and membrane permeability alterations. II. Phenotypic expression in intraspecific and interspecific somatic cell hybridsJournal of Cellular Physiology, 1979
- Coated pits, coated vesicles, and receptor-mediated endocytosisNature, 1979
- Selection of mouse x hamster hybrids using hat medium and a polyene antibioticIn Vitro Cellular & Developmental Biology - Plant, 1978
- Genetics of the LDL receptor: Evidence that the mutations affecting binding and internalization are allelicCell, 1977
- Toxicity of nystatin and its methyl ester toward parental and hybrid mammalian cellsIn Vitro Cellular & Developmental Biology - Plant, 1977
- RNA polymerase II regulation in α-amanitin-resistant rat myoblast mutants. Changes in wild-type and mutant enzyme levels during growth in α-amanitinJournal of Molecular Biology, 1977
- Isolation and partial characterization of a cholesterol-requiring mutant of Chinese hamster ovary cells.Proceedings of the National Academy of Sciences, 1977
- Sensitivity to Amphotericin B of in vitro Established Cell LinesNature, 1974
- Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro.Proceedings of the National Academy of Sciences, 1968