Rapid Identification of Probiotic Lactobacillus Biosurfactant Proteins by ProteinChip Tandem Mass Spectrometry Tryptic Peptide Sequencing
Open Access
- 1 February 2002
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (2) , 977-980
- https://doi.org/10.1128/aem.68.2.977-980.2002
Abstract
A novel ProteinChip-interfaced tandem mass spectrometer was employed to identify collagen binding proteins from biosurfactant produced by Lactobacillus fermentum RC-14. On-chip tryptic digestion of the captured collagen binding proteins resulted in rapid sequence identification of five novel tryptic peptide sequences via collision-induced dissociation tandem mass spectrometry.Keywords
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