Histochemical Detection of Acid and Alkaline Phosphatases in Periodontal Tissues After Experimental Tooth Movement

Abstract
A rubber dam was inserted between the 1st and 2nd molars on the left side of the upper jaw of 60 Wistar rats. The technique for the preparation of the tissue is described. The decreasing alkaline phosphatase activity of the peri-dontal ligaments on the pressure side may be due to biological alteration after orthodontic tooth movement and to a suppressing effect on certain metabolic activities associated with metabolic changes of periodontal fibers. The strongest acid and alkaline phosphatase activities in the calcifying ligaments may participate in calcification. Acid phosphatase in ligaments adjacent to the cementum was the same as controls. Acid phosphatase in the peridontal ligament is apparently not changed by tooth movement.

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