A SYSTEMATIC-APPROACH FOR DETECTING HIGH-FREQUENCY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS USING LARGE GENOMIC PROBES

  • 1 January 1985
    • journal article
    • research article
    • Vol. 37  (4) , 635-649
Abstract
Phage clones (13) containing low-copy sequences were isolated from a human DNA library and tested for their ability to detect restriction fragment length polymorphisms (RFLP). Reported are the RFLP revealed with each clone, all found in frequencies useful for linkage studies. Cytological data are available for 15 of the 13 clones, with regional assignments made for 3 of the markers by in situ hybridization. Phage clones containing large unique DNA inserts detect multiple RFLP with high efficiency. An analysis of the relative efficiency of 20 restriction enzymes for detecting single nucleotide changes is discussed by comparing the observed data to those expected on the basis of recognition and potential site frequencies, as computed from the dinucleotide distribution. In an effort to facilitate linkage studies using polymorphic DNA sequences, experiments were made with pools of probes from various sources.