Abstract
The stability of the thienamycin derivative MK0787 has been evaluated in the presence of β-lactamases from Staphylococcus aureus and Bacteroides fragilis, as well as the Types Ia, IIIa and IVc enzymes. In all cases the antibiotic was very stable. MK0787 also proved to be a potent suicide inhibitor of the Type Ia and Bacteroides fragilis enzymes. With pure Type IIIa β-lactamase the Km and Vmax values for MK0787 were found to be 67.μM and about 0 1 μmol antibiotic destroyed/μg/pure enzyme/min respectively.

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