Soybean Primary Leaves as a Site for Epiphytic Multiplication of Pseudomonas glycinea

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Abstract
A suspension containing 103 or 104 colony-forming units of either a motile or a nonmotile strain of P. glycinea [P. syringae] per ml was sprayed on the abaxial surface of soybean [Glycine max] primary leaves. The distribution of inoculum between the phylloplane and the mesophyll (epiphyllous and endophyllous inoculum, respectively) was determined immediately after inoculation. Colony counts up to 28 days after inoculation showed that bacterial populations derived from the total dose of inoculum were significantly greater than those developing from the endophyllous portion of inoculum only. Symptomless leaves and leaves bearing up to 4 bacterial blight lesions supported significantly higher populations arising from epiphyllous inoculum than did leaves with 5 such lesions.

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