THEOPHYLLINE RADIOIMMUNOASSAY - SYNTHESIS OF ANTIGEN AND CHARACTERIZATION OF ANTISERUM

  • 1 January 1976
    • journal article
    • research article
    • Vol. 13  (3) , 497-505
Abstract
Antisera to theophylline (T) were obtained by immunizing rabbits with a conjugate of 8-(3-carboxypropyl)-1,3-dimethylxanthine and bovine serum albumin. Comparison of 50% displacement values indicated good selectivity for T vs. a number of other xanthine derivatives. An analytical procedure using this antiserum can measure 200 pg of T and direct analysis of 0.1 .mu.g/ml in plasma or 0.02 .mu.g/ml in saliva is feasible.