Virus Detection by Nucleic Acid Hybridization: Examination of Normal and ALS Tissues for the Presence of Poliovirus
Open Access
- 1 October 1981
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 56 (2) , 223-233
- https://doi.org/10.1099/0022-1317-56-2-223
Abstract
A nucleic acid hybridization assay was developed as a sensitive assay for the presence of poliovirus RNA in human tissue. The assay could detect the presence of an average of 1 poliovirus/200 cells. A method for determining the extent of degradation of the tissue RNA was developed and used to show that a significant fraction of human CNS autopsy material contains highly degraded RNA which is unsuitable for hybridization studies. A total of 15 different control and amyotrophic lateral sclerosis (ALS) tissues were assayed for the presence of poliovirus-like RNA. Virus RNA was detected in 1 of the control tissues and in none of the ALS tissues.This publication has 3 references indexed in Scilit:
- Amyotrophic lateral sclerosisNeurology, 1980
- Sequence of picornavirus RNAs containing a radioiodinated 5'-linked peptide reveals a conserved 5' sequence.Proceedings of the National Academy of Sciences, 1980
- Amyotrophic lateral sclerosis, Parkinson's disease, and the amyotrophic lateral sclerosis-Parkinsonism-dementia complex on Guam: a review and summary of attempts to demonstrate infection as the aetiologyJournal of Clinical Pathology, 1972