Visualization of R-bands in human metaphase chromosomes by the restriction endonuclease MseI

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Abstract
Human metaphase chromosomes were treated with the restriction endonuclease Msel, which cuts DNA at TTAA sequences. This enzyme preferentially cuts and extracts DNA from G-bands and thus is the first restriction endonuclease allowing direct R-band visualization. Specific patterns ranging from R+C-like to C-like banding can be induced, depending on the concentration of the enzyme. At intermediate concentrations, only a subset of R-bands are produced, corresponding to GC-rich bands that are especially resistant to heat denaturation (so-called T-bands). These results suggest that compositional differences between chromosomal regions determine the different rates of cleavage by Msel, not only between R- and G-bands but also among different R-bands.

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