Effects of Hyperglycemia and Hyperthermia on the pH, Glycolysis, and Respiration of the Yoshida Sarcoma In Vivo2

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Abstract
Tissue (extracellular) pH (pHe) and intracellular pH (pHi) were measured together in vivo in the solid Yoshida sarcoma and normal organs (liver, gastrocnemius muscle) of noninbred Wistar rats. pHe was monitored by insertion of a miniature capillary glass electrode, and pHi was measured Indirectly by equilibrium partitioning of the weak organic acid 5,5-dimethyloxazolidine-2,4-dione across the cell membrane. Under normal conditions, tumor, liver, and gastrocnemius had a similar pHe of 7.05–7.30; tumor pHi was consistently higher (7.2) than that of the normal tissues (6.8–7.1). Curative hyperthermia (42° C for 1 hr) did not significantly change tumor pHe or pHi. After ip glucose injection [6 g/kg body wt, blood glucose level >400 mg/100 ml (22 mmoles/liter) for 4 hr], tumor pHe decreased markedly to 6.6 within 4 hours and did not return to normal for a further 12–14 hours, whereas tumor pHi was hardly affected. No marked change was noted in pHe or pHi of the normal organs following glucose loading of the host. In tumor slices removed from hyperglycemic hosts, marked reduction of both respiration and glycolysis was observed. Hyperglycemia (4 hr) plus hyperthermia at 40° C (1 hr) had a synergistic inhibitory effect on metabolism that was equivalent to heat alone at 42° C, and respiration and glycolysis almost ceased after 3–4 hours. However, tumor heating at 40° C in hyperglycemic hosts was not equivalent to hyperthermia at 42° C: With the former treatment, tumor regression did not occur, and animal survival did not differ from that of control untreated rats. The data do not support the postulate that the effects of heat on tumor cells are mediated via low pHi or that hyperglycemia leads to a lowered pHi which sensitizes the tumor to destruction at 40° C instead of 42° C.