A Microdilution Method for Antibiotic Susceptibility Testing: An Evaluation

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Abstract
A micromethod for serial dilution antibiotic susceptibility tests has been described and evaluated. The delivery of optimal-sized droplets of medium and inoculum is influenced greatly by static electric charges on plastic well plates in which the test is performed. Dissipation of these charges by ultraviolet irradiation improved the results so that minimum inhibitory concentration (MIC) end-points were comparable within plus or minus one dilution step, when compared with conventional tube dilution susceptibility tests. Further comparison showed the reproducibility of the micromethod to be only slightly less than that of the conventional tube dilution technic. Once the user is aware of certain pitfalls inherent in the microdilution method, it can provide reliable results.

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