Structure of the alpha-fetoprotein gene in the mouse.
- 1 March 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (3) , 1351-1355
- https://doi.org/10.1073/pnas.77.3.1351
Abstract
The mouse .alpha.-fetoprotein mRNA is the product of a single-copy gene whose mRNA coding sequences are represented discontinuously in the genome. Several EcoRI genomic fragments which contain portions of the .alpha.-fetoprotein gene were cloned by using the EK2 vector .lambda.gt WES .cntdot. .lambda.B. In addition, a mouse genomic library was screened to obtain a 15.75-kilobase segment of DNA that includes more than 85% of the .alpha.-fetoprotein coding sequence. Analyses by restriction endonuclease mapping and EM showed that the mRNA sequence is interrupted by at least 11 intervening sequences which occupy 90% of the cloned DNA.Keywords
This publication has 29 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- A comparison of two cloned mouse β-globin genes and their surrounding and intervening sequencesCell, 1978
- In vitro packaging of a λ Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1Gene, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- EK2 Derivatives of Bacteriophage Lambda Useful in the Cloning of DNA from Higher Organisms: The λgt WES SystemScience, 1977
- α Foetoprotein and serum albumin show sequence homologyNature, 1976
- Alpha‐fetoprotein: Immunochemical Purification and Chemical Properties. Expression in Normal State and in Malignant and non‐Malignant Liver DiseaseImmunological Reviews, 1974
- alpha-Fetoprotein as a Marker of Embryo-Specific Differentiations in Normal and Tumor TissuesImmunological Reviews, 1974
- Embryonal serum α‐globulin in cancer patients: Diagnostic valueInternational Journal of Cancer, 1967
- Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.Journal of Clinical Investigation, 1966