Sequence analysis of a cloned cDNA coding for bovine seminal ribonuclease
Open Access
- 1 October 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 152 (2) , 275-277
- https://doi.org/10.1111/j.1432-1033.1985.tb09194.x
Abstract
The sequence of a cloned cDNA coding for bovine seminal ribonuclease, an enzyme secreted in the bull seminal vesicles, was determined. The cDNA starts at the amino acid residue 47 and terminates 12 nucleotides beyond the consensus sequence AAUAAA in the 3′ non-coding region of the mRNA. Northern blotting analysis shows that the mRNA for bovine seminal ribonuclease consists of about 950 nucleotides, a value that is similar to that of other mRNAs coding for ribonucleases of the pancreatic type.This publication has 14 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Working of the genetic codeTrends in Biochemical Sciences, 1980
- A rapid and efficient procedure for the purification of DNA from agarose gelsAnalytical Biochemistry, 1980
- RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexaminationBiochemistry, 1977
- Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proceedings of the National Academy of Sciences, 1975
- Dimeric structure of seminal ribonucleaseFEBS Letters, 1972
- Bull Semen Ribonucleases. 1. Purification and Physico-Chemical Properties of the Major ComponentEuropean Journal of Biochemistry, 1972
- 24 Bovine Pancreatic RibonucleasePublished by Elsevier ,1971
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970