A histochemical study of glycogen metabolism in developing cartilage and bone

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Abstract
The distribution of glycogen, phosphorylase, and glycogen synthetase was studied during the early development of cartilage and bone in the chick. The relative amounts of each of these substances was related to cellular activity. Cellular activity was assessed by measuring the rate of matrix deposition and by a study of the ground substance with the toluidine blue and alcian blue histochemical methods. In both cartilage and bone, there is a close correlation between the relative amounts of glycogen within the cells and the rate of matrix deposition. Also, the intensity of the phosphorylase reaction parallels the use of glycogen by the cells. Glycogen synthetase gave the most intense reaction during the early stages of chondrification but could not be demonstrated, with certainty, during the ossification process. In both cartilage and bone there appears to be a direct correlation between cellular activity, in terms of matrix synthesis, and the synthesis and degradation of glycogen by the cells.

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