Interleukin‐HP1‐related hybridoma and plasmacytoma growth factors induced by lipopolysaccharide in vivo

Abstract
Serum of lipopolysaccharide (LPS)‐treated mice was found to support the growth of interleukin‐HP1 (HP1)‐dependent hybridoma and plasmacytoma cell lines. This growth‐promoting activity, which was undetectable in normal serum, rose more than 1000‐fold within 2 h after i.v. injection of the toxin and disappeared in less than 1 day. It could be traced to a single component, which behaved like HP1 in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and reverse phase high performance liquid chromatography, and was completely inhibited by a rabbit anti‐HP1 antiserum. The LPS‐induced factor was apparently not of T cell origin, as indicated by the strong activity found in the serum of LPS‐treated nude mice. In vitro, LPS also considerably enhanced the production by macrophages of a factor with similar characteristics.

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