Generation of a Hybrid Sequence-Specific Single-Stranded Deoxyribonuclease
- 4 December 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 238 (4832) , 1401-1403
- https://doi.org/10.1126/science.3685986
Abstract
The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3'-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.This publication has 29 references indexed in Scilit:
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