METABOLISM OF TESTOSTERONE-3-C14IN THE GUINEA PIG1

Abstract
ANDROSTERONE and 3α-hydroxyetiocholan-17-one have been demonstrated to be the principal urinary metabolites of administered testosterone in man (Callow, 1939; Dorfman et al., 1939; Fukushima et al., 1952; and West et al., 1951). Epiandrosterone has been isolated from the urine of a male guinea pig (Dorfman and Fish, 1940) and of a hypogonadal man (Dorfman, 1941) following the administration of testosterone. Since the guinea pig is being used for steroid hormone studies, it would be of interest to know the principal urinary metabolites of testosterone in this species. To this end, testosterone-3-C14 was administered intraperitoneally to a male guinea pig and by the addition of suitable carrier steroids to the urinary extracts, several metabolities of testosterone have been demonstrated. experimental 6 mg. (1.2×106 cts./min./mg.)2 of testosterone -3-C14 prepared by the method of Turner (1950) were administered in corn oil to an 800 gm. male guinea pig.
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