An alloantibody against a class II antigen subtypic to HLA-DR4 and strongly associated with the cellularly defined Dw14 determinant

Abstract
A pregnancy-induced alloantibody has allowed definition of a class II HLA antigen, AE, which has a frequency of about 10% in Danes, is completely included in DR4, and highly significantly (p = 10-9) associated with the cellularly defined Dw14 determinant which is completely included in AE. AE is absent on Dw4 and Dw13 cells, but was also found on one Dw15 HTC and in two of four Dw10-positive cells. It is in positive linkage disequilibrium with B40 but not with B15 or B44. It is hypothesized that the AE specificity is due to the co-occurrence of an arginine in position 71 and an alanine in position 86 of the first domain of the DR.beta.1 chain. However, it cannot be excluded that this antigen belong to another class II series.

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