Cellular Origin of the Chemokinetic Inhibitor of Polymorphononuclear Leucocytes Found in Sera from Patients with Chronic Lymphocytic Leukaemia

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Abstract
In a previous study, we demonstrated the existence in serum from patients with chronic lymphocytic leukaemia (CLL) of a cell‐directed, heat‐labile chemokinetic inhibitor of polymorphonuclear leucocytes (PMN). The aim of this investigation was to study the cellular origin of this inhibitor. Supernatants from short term cultured lymphocytes from 3 healthy persons and 5 patients with CLL, and from 16 different established human non‐haematopoietic and haematopoietic cell lines were analyzed. Only the leukaemic B‐lymphocytes from the CLL patients produced the inhibitor. The presence of the inhibitor was also demonstrated in supernatants from the fresh CLL cells from one CLL patient and a leukaemic cell line established from these cells using Epstein‐Barr virus as the immortalizing agent. A lymphoblastoid cell line derived from the normal B‐cells of the same CLL patient did not produce the chemokinetic inhibitor. We therefore conclude that the cellular origin of the heat‐labile, cell‐directed chemokinetic inhibitor of PMN migration is the CLL cell.