ISOLATION AND CHARACTERIZATION OF A LARGE CELL POSSIBLY POLYPLOID STRAIN OF ESCHERICHIA COLI
- 1 October 1957
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 74 (4) , 477-484
- https://doi.org/10.1128/jb.74.4.477-484.1957
Abstract
When cells of a purine-requiring mutant of E. coll were cultivated in an atmosphere of camphor vapors, a stable large cell strain was produced which exhibited no significant change in growth rate of fermentation characteristics from the parent strain. An increase in the dry weight, desoxy-ribonucleic acid and ribonucleic acid content, plus the presence of more chromatin-staining bodies per cell, suggest that the large cell strain is diploid or polyploid. However, comparative frequencies of radiation-induced T1 phage resistance and streptomycin resistance mutations are at variance with the diploid or polyploid hypothesis.Keywords
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