Optimization of the isocratic non‐aqueous reverse phase (NARP) HPLC separation of trans/cis‐α‐and β‐carotenes

Publisher Website
Google Scholar
Abstract
Non‐aqueous reverse phase chromatography was used to optimize the separation of all‐trans‐α‐ and β‐carotenes and their cis isomers extracted from dried carrots. The technique can easily be applied to routine assays. Optimization was carried out by modifying the concentrations of methylene chloride and acetonitrile, and by studying the influence of temperature on the capacity factors and selectivity. The effect of the type of C 18 stationary phase bonding (mono‐ and polyfunctional) on separation was also examined. The need for polymeric bonding on the stationary phase is stressed for the realization of the trans/cis isomer separation.