4-DESOXYHUMULONES IN HOPS

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Abstract
Previous studies on the 4-desoxyhumulones in hops using classical methods indicated that these substances may account for up to 0·05% of mature Hallertau hops. 4-Desoxycohumulone and 4-desoxyhumulone are now shown to be detectable directly, and simultaneously with the α-acids and β-acids, by gas-liquid chromatography of their trimethylsilyl ethers; identification has been confirmed by mass-spectrometry. Quantitative analysis is possible by use of standard 4-desoxycohumulone, conveniently prepared by ultraviolet irradiation of colupulone. This indicates that the concentration of the 4-desoxyhumulones is in fact around 0·3% of mature hops—seven to ten times the previously reported concentration.

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