Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex
Top Cited Papers
- 10 February 2008
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 5 (3) , 235-237
- https://doi.org/10.1038/nmeth.1184
Abstract
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.Keywords
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