Similar specificity fo IgG1 and IgG2a antibodies in individumice

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Abstract
In this study the previous finding of similar variable regions in individual IgG1 and IgG2a antibody populations is extended by the demonstrations of similar fine specificity of IgG1 and IgG2a combining sites. Antibody populations form individual mice directed against oligo‐D‐alanine determinants were analyzed in their cross‐reactivityb towards 5 heterologous dipeptides. This was done by mixing antibody and hapten followed by determination of free antibodies in a kinetic red cells sensitization assay. The comparison of hyperimmune sera fror 10 mice showed that genetically identical mice can differ significantly in their cross‐reaction pattern. Within each serum the cross‐reaction pattern was determinated for IgG1 and IgG2a. With a few exceptions the same individual pattern was found in both IgG1 and IgG2a antibody populations. This was taken as evidence that the combining sites of IgG1 and IgG2a anti‐oligo‐D‐alanine antibody populations in an individual mouse are similar.