Biotin Transport and Accumulation by Cells of Lactobacillus plantarum II. Kinetics of the System

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Abstract
Bound biotin-saturated cells of L. plantarum accumulated free biotin by a time-dependent process exhibiting substrate saturation phenomena in the presence and absence of glucose. Apparent Km and Vmax values determined in the presence and absence of glucose, respectively, from Lineweaver-Burk plots were found to be 31.5 and 7.72 mjiM (Km) and 9.72 and 3.26 MMmoles per mg per min (Vmax). Free biotin transport per. se appeared to be an energy-independent, mediated process, whereas the accumulation of large intracellular vitamin concentrations was energy-dependent. Internal free biotin was quantitatively converted to bound biotin. The rate and extent of bound biotin formation was slower than free biotin uptake, and dependent upon intracellular free biotin levels up to a saturating concentration.

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