Effects of Freeze-Thawing and Storage on Ultracentrifugal Properties of Human Serum Lipoproteins

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Abstract
A comparative study of the effects on human sera of storage at 3 ranges of temperature showed that adequate preservation of both low and high density lipoprotein fractions is obtained for approximately 4 weeks at temperatures between -5[degree] and 0[degree]C, 2 weeks at temperatures between 0[degree] and +4[degree]C, and only a few days at temperatures between -30[degree] and -26[degree]C. Storage at temperatures between -30[degree] and -25[degree]C involved rapid and slow freeze-thawing processes. Preliminary experiments indicated no significant lipoprotein changes after successive cycles (from 1 to 3 times) of either rapid or slow freeze-thawing of serum.