STUDIES ON IMMUNE HUMAN HEMOLYSIS
Open Access
- 1 January 1961
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 113 (1) , 177-192
- https://doi.org/10.1084/jem.113.1.177
Abstract
Studies on the kinetics of the Donath-Landsteiner reaction, using purified antibody, human complement, and human erythrocytes, indicate that the requirement for complement specifically in the cold or the warm phase of the reaction is a function of antibody potency, erythrocyte sensitivity, and environmental conditions. Optimal conditions include 1[degree]C for cold phase, 32[degree]C for warm phase, pH 7.4, ionic strength 0.15. Ca++ is required in the cold phase and Mg++ in the warm phase of the reaction.Keywords
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