Evaluation of an Enzymatic Method For Determination of Glucose In Whole Blood Using Flow Injection Analysis With Detection By Chemiluminescence

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Abstract
A flow injection system for the determination of D-glucose using on-line dialysis is described and its potential use for whole blood measurements is evaluated. Glucose is degraded enzymatically by means of glucose oxidase which is immobilized on controlled porosity glass and incorporated into a small PVC column reactor. the hydrogen peroxide generated by the glucose oxidase is determined by chemiluminescence with an alkaline reagent containing luminol and hexacyanoferrate (III). the detection limit was 0.1 mM glucose with a sampling frequency of 95 h-1 and a standard deviation of less than 1.5%. the enzyme reactor showed little change in activity over a 6 months period of operation. the use of an open tubular nylon coil, in which the glucose oxidase is immobilized to the wall, is also described.