Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein
Open Access
- 18 December 2001
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (26) , 14837-14842
- https://doi.org/10.1073/pnas.201399298
Abstract
Recombinant Escherichia coli cells engineered for the expression of the xylB gene in conjunction with genes of the nonmevalonate pathway were supplied with 13 C-labeled 1-deoxy- d -xylulose. Cell extracts were analyzed directly by NMR spectroscopy. 13 C-labeled 2 C -methyl- d -erythritol 2,4-cyclodiphosphate was detected at high levels in cells expressing xylB , ispC , ispD , ispE, and ispF . The additional expression of the gcpE gene afforded 1-hydroxy-2-methyl-2-( E )-butenyl 4-diphosphate as an intermediate of the nonmevalonate pathway. Hypothetical mechanisms involving conserved cysteine residues are proposed for the enzymatic conversion of 2 C -methyl- d -erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-( E )-butenyl 4-diphosphate catalyzed by the GcpE protein.Keywords
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