An Investigation of the Kinetic Characteristics of the Lipase from Candida cylindracea for Its Potential in Triglyceride Analysis
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 12 (1) , 47-69
- https://doi.org/10.1080/00032717908082518
Abstract
Optimum reaction conditions by pH-stat studies of lipase from candida cylindracea towards triolein emulsions were determined. These conditions are no less than 40 mM calcium ion and no more than 0.3 M sodium chloride in 10 mM (tris-hydroxymethyl) amino methane (THAM) buffer at pH 7.45. Comparison of the enzyme activity and relative amount of product formed as a function of calcium ion concentration imply a thermodynamic as well as a kinetic role for calcium. Increasing the THAM concentration to 0.5 M appears to facilitate the deactivation of the enzyme by the free fatty acid produced in the reaction.Keywords
This publication has 13 references indexed in Scilit:
- Analytical aspects of immobilized enzyme columnsAnalytical Chemistry, 1977
- Competitive inhibitory effect exerted by bile salt micelles on the mydrolysis of tributyrin by pancreatic lipaseBiochemical and Biophysical Research Communications, 1976
- Further studies on the exocellular lipase of Rhizopus arrhizusBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
- On the positional and chain specificities of Candida cylindracea lipaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1971
- Action of some effectors on the hydrolysis of long-chain triglycerides by pancreatic lipaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968
- Sur le role des ions calcium durant l'hydrolyse des triglycerides insolubles par la lipase pancreatique en presence de sels biliaresBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- Studies on Lipase from Candida cylindraceaAgricultural and Biological Chemistry, 1966
- Studies on Lipase from Candida cylindraceaAgricultural and Biological Chemistry, 1966
- Action de la lipase pancréatique sur les esters en émulsionBiochimica et Biophysica Acta, 1958
- On the mechanism of pancreatic lipolysis of glyceridesBiochimica et Biophysica Acta, 1954