Covalently bound pyruvate in phosphopantothenoylcysteine decarboxylase from horse liver
- 9 February 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 212 (1) , 79-82
- https://doi.org/10.1016/0014-5793(87)81560-0
Abstract
Horse liver phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) incorporates nonexchangeable tritium from borotritide with a decrease of the activity. Substrate prevents both tritium incorporation and the decrease in activity. Acid and base hydrolysis of the tritiated protein releases labeled lactate identified by highvoltage paper electrophoresis, paper chromatography and silicic acid chromatography. These results indicate the presence of pyruvate covalently bound through an ester linkage to phosphopantothenoylcysteine decarboxylase which is then another example of a mammalian enzyme in which pyruvate is involved in a catalytic activity.Keywords
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