SPECIES DIFFERENCE IN ELECTROPHORETICALLY DISTINCT FORMS OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE IN SOME MAMMALS

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Abstract
A comparative study was undertaken on glucose 6-phosphate dehydrogenase (G6PD) extracted from various organs of mice, rats, hamsters and guinea pigs, and from human red blood cells which were separated into several molecular forms by means of acrylamide disc electrophoresis. Two major forms of G6PD were demonstrated in each animal. The one located mainly in microsomal fraction moved slowly and was resistant to inhibitors. The other, located in cell sap as well as in mitochondria, moved rapidly and was sensitive to inhibitors. The latter form seems to be homologous to the sex-linked G6PD of human red cells. Isoenzyme pattern showed sex differences in the rat liver and in the mouse plasma. In the rat liver, band D enzyme was more active in females than in males, while in the mouse plasma, band I enzyme was more active in males than in females. Both bands exhibited the same electrophoretic mobility.