Quantitative Analysis of Emetine and Cephaeline by Reversed-Phase High Performance Liquid Chromatography with Fluorescence Detection
- 1 March 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 8 (2) , 63-65
- https://doi.org/10.1093/jat/8.2.63
Abstract
A versatile method for the quantitation of emetine and cephaeline in biological samples is described. Two milliliters of samples containing N-propylprocainamide as the internal standard are buffered to pH 9 and extracted with n-butyl chloride. After subsequent back extraction into 0.01M hydrochloric acid, a portion of the acid layer is analyzed by reversed-phase high performance liquid chromatography with fluorescence detection. Routinely, the minimum level of detection for both drugs is 5 ng/mL and linearity is demonstrated from 5 to 2500 ng/mL.This publication has 2 references indexed in Scilit:
- Determination of emetine and cephaeline in Ipecac roots by high- performance liquid chromatographyJournal of Chromatography A, 1982
- Comparative Pharmacokinetic Studies of Dehydroemetine and Emetine in Guinea Pigs Using Spectrofluorometric and Radiometric Methods *The American Journal of Tropical Medicine and Hygiene, 1965