Envelope-associated nucleoid from Caulobacter crescentus stalked and swarmer cells

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Abstract
Envelope-associated nucleoids were isolated from C. crescentus by using a modification of a previously described procedure. The development of a Ludox density gradient procedure permitted preparation of large quantities of synchronous cells. The sedimentation coefficients of the envelope-associated nucleoids of stalked and swarmer cells, prepared under conditions of equivalent cell lysis, were 3000 S and > 6000 S, respectively. Small differences in the relative amounts of DNA, RNA and protein in stalked and swarmer cell envelop-associated nucleoids could not account for the large differences in sedimentation behavior. These characteristic sedimentation coefficients were retained in mixing experiments.