Intramembrane Proteolysis of Signal Peptides: An Essential Step in the Generation of HLA-E Epitopes
- 1 December 2001
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 167 (11) , 6441-6446
- https://doi.org/10.4049/jimmunol.167.11.6441
Abstract
Signal sequences of human MHC class I molecules are a unique source of epitopes for newly synthesized nonclassical HLA-E molecules. Binding of such conserved peptides to HLA-E induces its cell surface expression and protects cells from NK cell attack. After cleavage from the pre-protein, we show that the liberated MHC class I signal peptide is further processed by signal peptide peptidase in the hydrophobic, membrane-spanning region. This cut is essential for the release of the HLA-E epitope-containing fragment from the lipid bilayer and its subsequent transport into the lumen of the endoplasmic reticulum via the TAP.Keywords
This publication has 47 references indexed in Scilit:
- In search of the ‘missing self’: MHC molecules and NK cell recognitionPublished by Elsevier ,2003
- A Role for a Novel Luminal Endoplasmic Reticulum Aminopeptidase in Final Trimming of 26 S Proteasome-generated Major Histocompatability Complex Class I Antigenic PeptidesPublished by Elsevier ,2001
- Release of Signal Peptide Fragments into the Cytosol Requires Cleavage in the Transmembrane Region by a Protease Activity That Is Specifically Blocked by a Novel Cysteine Protease InhibitorJournal of Biological Chemistry, 2000
- Regulated Intramembrane ProteolysisCell, 2000
- DEGRADATION OF CELL PROTEINS AND THE GENERATION OF MHC CLASS I-PRESENTED PEPTIDESAnnual Review of Immunology, 1999
- NK CELL RECEPTORSAnnual Review of Immunology, 1998
- MECHANISMS OF MHC CLASS I–RESTRICTED ANTIGEN PROCESSINGAnnual Review of Immunology, 1998
- Improved electrophoretic separation and immunoblotting of beta‐amyloid (Aβ) peptides 1–40, 1–42, and 1–43Electrophoresis, 1997
- Protein insertion into the endoplasmic reticulum of permeabilized cellsFEBS Letters, 1995
- Signal Sequence Recognition and Protein Targeting to the Endoplasmic Reticulum MembraneAnnual Review of Cell and Developmental Biology, 1994