Isolation of a Caulimovirus from Strawberry Tissue Infected with Strawberry Vein Banding Virus

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Abstract
Strawberry vein banding virus (SVBV), a semipersistent aphid-transmitted virus, was partially purified from infected strawberry tissue utilizing a step gradient consisting of a layer of 1.50 g/cm3 CsCl in 30% sucrose. Semipurified SVBV collected from the gradient interface reacted heterologously with antiserum to the cabbage B strain of cauliflower mosaic virus (CaMV). Sedimentable antigenic activity was detected by ELISA [enzyme linked immunosorbent assay] in the 200S region of linear log sucrose gradients for both SVBV and CaMV after treatment with formaldehyde. Chromatography of SVBV and CaMV on ECTEOLA (Cellex-E) ion exchange cellulose revealed that both eluted at approximately 0.18 M NaCl. Spherical virions about 45 nm in diameter were seen by serologically specific EM of semipure SVBV and CaMV extracts and typical caulimovirus cytoplasmic inclusions were observed by EM in ultrathin sections of SVBV-infected strawberry leaves. SVBV is a legitimate member of the caulimovirus group.