Application of 24-Hour Postexposure Observation to Acute Toxicity Studies with Invertebrates

Abstract
Experimentation with carbaryl, dieldrin, endothal, malathion, and TFM (3-trifluoromethyl-4-nitrophenol) on the ostracod Cypretta kawatai and the midge Chironomus tentons demonstrated that the method of routine toxicity tests with invertebrates can be substantially improved by including a posttreatment (toxicant-free water) observation period. This improvement enables confirmation of death and thereby reveals latent toxicity or recovery.