Method of Chorioallantoic Membrane Inoculation which Decreases Nonspecific Lesions

Abstract
The artificial air space was rotated to a new choriallantoic membrane area after its formation. The rotation was made to reduce the occurrence of nonspecific lesions in the inoculation area. A statistical assessment of this method with variola virus titrations gave a significantly higher mean value and a 66% reduction of nonspecific lesions as compared to a non-rotated method. The advantages and application of this technique are dis-cussed.

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