A cryoglue to mount vitreous biological specimens for cryoultramicrotomy at 110K

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Abstract
Mixtures of ethanol, 2‐propanol and 2‐butanol can be used as a cryoglue to mount vitrified biological specimens for ultrathin cryosectioning. Brought directly from room temperature to a cutting support at 140K in the cold chamber of a cryoultramicrotome, these alcohols stiffen to a viscous and gluey consistency allowing the attachment or embedding of a vitreous biological sample. The mass hardens at lower temperatures fixing the sample well for microtomy. With ethanol: 2‐propanol (2:3), samples are applied at 140 K and ultrathin cutting can be done at 115 K.