Characterization of collagen‐like heterotrimers: Implications for triple‐helix stability

Abstract
This article deals with the effects of proline hydroxylation on collagen triple‐helix stability, an issue that is still under discussion. To investigate the structural determinants of triple‐helix stabilization by hydroxyproline (Hyp), we here characterized spectroscopically triple‐helix heterotrimers containing both chains of (Pro–Pro–Gly)10 and (Pro–Hyp–Gly)10. Results are discussed in relation to the various triple‐helix stabilization mechanisms. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004