Short Communications. Evidence for an active dimer of Escherichia coli β-galactosidase
- 1 November 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 151 (2) , 433-434
- https://doi.org/10.1042/bj1510433
Abstract
β-Galactosidase (EC 3.2.1.23), prepared from strains ML 308 and K12 3300 of Escherichia coli, dissociated into an inactive monomer in the presence of Ag+. When such a monomer preparation is treated with excess of thiol an enzymically active dimer is formed in addition to an active tetramer. It is suggested that Ag+ may be of value in studies on other multimeric proteins as a mild dissociating agent.Keywords
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