THE MEDIATION OF TISSUE EOSINOPHILIA IN HYPERSENSITIVITY REACTIONS .4. PRODUCTION OF DELAYED EOSINOPHIL CHEMOTACTIC FACTOR-A BY PERITONEAL-EXUDATE CELLS FROM SENSITIZED GUINEA-PIGS INVITRO

  • 1 January 1983
    • journal article
    • research article
    • Vol. 48  (1) , 69-77
Abstract
An eosinophil chemotactic factor (ECF) was produced in the cell-free culture supernatants (CFS) of peritoneal exudate cells (PEC) from guinea pigs immunized with dinitrophenyl derivatives of ascaris extract (DNP-As) when stimulated by the antigen in vitro without activation by immune complexes. A 2- or 6-h pulse of the antigen was sufficient for ECF production; long time incubation (48 h) was required for the production of a sufficient amount of the factor. Treatment of PEC by cycloheximide resulted in the reduction of ECF production, suggesting that protein synthesis is essential. Its generation appeared carrier-specific, and the source of the factor is presumed to be lymphocytes, probably T lymphocytes. The factor, with a MW of 70,000, shared a common antigenicity with delayed ECF-a, which was isolated from the skin lesions showing delayed tissue eosinophilia in vivo.