A Distinct Group of Hepacivirus/Pestivirus-Like Internal Ribosomal Entry Sites in Members of DiversePicornavirusGenera: Evidence for Modular Exchange of Functional Noncoding RNA Elements by Recombination

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Abstract
The 5′ untranslated regions (UTRs) of the RNA genomes ofFlaviviridaeof theHepacivirusandPestivirusgenera contain internal ribosomal entry sites (IRESs) that are unrelated to the two principal classes of IRESs ofPicornaviridae. The mechanism of translation initiation onhepacivirus/pestivirus (HP) IRESs, which involves factor-independent binding to ribosomal 40S subunits, also differs fundamentally from initiation on these picornavirus IRESs. Ribosomal binding to HP IRESs requires conserved sequences that form a pseudoknot and the adjacent IIId and IIIe domains; analogous elements do not occur in the two principal groups of picornavirus IRESs. Here, comparative sequence analysis was used to identify a subset of picornaviruses from multiple genera that contain 5′ UTR sequences with significant similarities to HP IRESs. They are avian encephalomyelitis virus, duck hepatitis virus 1, duck picornavirus, porcine teschovirus, porcine enterovirus 8, Seneca Valley virus, and simian picornavirus. Their 5′ UTRs are predicted to form several structures, in some of which the peripheral elements differ from the corresponding HP IRES elements but in which the core pseudoknot, domain IIId, and domain IIIe elements are all closely related. These findings suggest that HP-like IRESs have been exchanged between unrelated virus families by recombination and support the hypothesis that RNA viruses consist of modular coding and noncoding elements that can exchange and evolve independently.

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