Incorporation of Labeled Amino Acid into Protein by Thyroid Glands from Hypophysectomized Rats: I.In VitroStudies
- 1 December 1971
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 89 (6) , 1477-1484
- https://doi.org/10.1210/endo-89-6-1477
Abstract
The incorporation of labeled amino acid into different protein fractions has been studied in the thyroid glands from normal and hypophysectomized rats after incubation in vitro. The absolute amount of labeled thyroglobulin decreases by 58–60% (p < 0.001) after hypophysectomy. Under identical experimental conditions the labeling of the other protein fractions studied (total proteins, particulate and soluble proteins other than thyroglobulin) remained unchanged in the glands from operated animals. In the medium containing low concentration of labeled amino acid (8 μg) this decrease does not become evident until the incubations with 3H-Tyr have been carried out for 8 or more hours. The decrease of thyroglobulin labeling is however, very significant from the shortest time of incubation (2 hr) if the glands are incubated in the medium containing higher concentration of 3H-Tyrosine (30 μg). (Endocrinology89: 1477, 1971)Keywords
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