THYMIDINE AND HYPOXANTHINE REQUIREMENTS FOR THE PROLIFERATION OF NORMAL AND ROUS-SARCOMA VIRUS-INFECTED CHICKEN FIBROBLASTS IN THE PRESENCE OF METHOTREXATE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (5) , 1854-1856
Abstract
Cultured normal and Rous sarcoma virus-infected chicken fibroblasts do not differ in the concentrations of thymidine or hypoxanthine that they require to proliferate in the presence of a methotrexate [an antineoplastic drug] block. For maximal proliferation, thymidine is required at 10-6 M, while hypoxanthine is required at 10-5 M. The normal and Rous-infected fibroblasts show very similar, if not identical, decreases in proliferation rates at suboptimal concentrations of thymidine or hypoxanthine. Conversion of fibroblasts to the neoplastic state apparently does not alter their capacity to salvage thymidine or purines from the extracellular fluid or to metabolize these compounds.

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