Physiological characteristics of Trebouxia (Chlorophyceae, Chlorococcales) and Pseudotrebouxia (Chlorophyceae, Chlorosarcinales)

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Abstract
The phycobionts of Trebouxia (Chlorococcales) and of Pseudotrebouxia (Chlorosarcinales) grow well on 1·5–1·0% agarized Bold's Basal Medium. Vitamins are not necessary for maintaining growth. Glucose enhances growth in liquid medium. Utilization of inorganic and organic sources of nitrogen varies between genera and among Isolates of the same species from different lichens. Preference for a specific nitrogen source in Trebouxia may be correlated with the lichen from which a species was isolated. Urea inhibits growth of all species of both genera tested. Carbon assimilation varies from species to species between the two genera. No special pattern of carbon utilization between the genera is apparent except that Pseudotrebouxia utilizes fructose better than glucose. Trebouxia prefers glucose. Heterotrophic growth in the 6-carbon sugars is exhibited by several species of both genera. Some can utilize sodium acetate.

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