Preliminary crystallographic studies of an anti‐HIV‐1 protease antibody that inhibits enzyme activity

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Abstract
F11.2.32, a monoclonal antibody directed against the HIV-1 protease, displays strong inhibitory effects toward the catalytic activity of the enzyme. The antibody cross-reacts with peptides 36–46 and 36–57 from the protease. Crystals of the Fab have been obtained both in the free state and as complexes formed with the protease peptide fragments, 36–46 and 36–57. Diffraction data have been collected for the free and complexed forms of Fab F11.2.32 and preliminary models for the crystal structures were obtained by molecular replacement.
Funding Information
  • Institut Pasteur
  • Centre National de la Recherche Scientifique
  • Fondation pour la Recherche Médicale
  • European Commission
  • Grant Agency of the Czech Republic
  • Biochemical Fund